第一篇：向老外索要文章或試劑的

Dear Mr./Mrs.: ________（Author name）

I am a graduate student of Harbin Medical University in China.I major in “________”（您的專業(yè)）.Recently, I found one of your articles, titled “__________”（Title）in Medline.I found it may help me achieve my goals in this research field.This would make a really positive contribution to my work.I would like to be able to read the full text of this article.The abstract makes the article sound very interesting.I know there is usually a fee required to obtain the full article from Medline;however, as a student, my only income is a small scholarship which is about U S $30.00 per month.I wonder if you would consider sending me the full text by Email.Perhaps you would consider this as an act of friendship between our two countries.Thank you for your kind consideration of this request.Sincerely: ___________（your name）

My Email address is: ____________________（your email）

Date：Month/day/year

Dear professor ***，I appreciate that you can take the time to read this e-mail.I am ***, a post-graduate student in the School of the ***, *** University in China.I am very interested in ***.We had got several mutants about*** gifted by *** in ***.Those mutants have been used to explain***.However, in my recent trial,***.In your article “***”, you isolated the strain*** and confirmed that ***.This special experimental phenomenon was very similar to that I observed.So I doubted the role of *** may involve in the strange phenomenon in my study.Because of lacking the plasmids and strains used for genetic modification of ***, it is difficult for us to construct the relevant mutants used in your articles.Therefore, I am writing to you to seek your help.Can you kindly provide us those mutants including****? It would be very helpful to us.Thank you very much in advance.Yours sincerely，Dear Professor Bruss V: I am working on immunology.And one of my projects focuses on the treatment of HBV.We want to construct a vector pHBV1.5 which your used in one of your paper(Proc.Natl.Acad.Sci.USA,vol88:1059-1063).Construct this vector seems to be a problem hard to get over for us.One of my graduates has been working on it for a couple of months, but no progress.We are happy to find that your laboratory was the major source of the pHBV1.5.Therefore, I am writing to explore the possibility of getting pHBV1.5 from your laboratory.If you were kindly offering us the vector, it would be very helpful for us.Of course, the plasmid will never be used for commercial purpose.We will acknowledge the plasmid resource and cite your papers in all our publications based on the plasmid.Sure, We would like to sign any contract for the plasmid transfer.One of my graduates is waiting for the vector to set up his experiment, so your early response will be greatly appreciated.Dear Mr./Mrs.: ________（作者名）

I am a graduate student of Harbin Medical University in China.I major in “________”（您的專業(yè)）.Recently, I found one of your articles, titled “__________”（文章名）in Medline.I found it may help me achieve my goals in this research field.This would make a really positive contribution to my work.I would like to be able to read the full text of this article.The abstract makes the article sound very interesting.I know there is usually a fee required to obtain the full article from Medline;however, as a student, my only income is a small scholarship which is about U S $30.00 per month.I wonder if you would consider sending me the full text by Email.Perhaps you would consider this as an act of friendship between our two countries.Thank you for your kind consideration of this request.Sincerely: ___________（您的名字）My Email address is: ____________________（你的email地址）

Dear Prof.XXX: I am in...............P.R.China.I am writing to request your assistance.I search one of your papers: “.......................” is published at................., Volume.., Issue..年, Pages..............but I can not read full-text content, would you mind sending your papers by E-mail? Thank you for your assistance.Best wishes!Dear prof i am very interested in you paper “cell, 2007, 34, 2341”.But i cannot get the fulltext.would u pls send me a pdffulltext? thanks

Dear ×××, I'm a researcher in ________（國(guó)家或大學(xué)）and just read an abstract of your paper “XXXXXXXXXXXX” I'm very interested to know ________（對(duì)方文章內(nèi)容）.It would be much appreciated if you can reply me about this.Also, could you please send me the copy of your full paper.Thanks in advance.Best regards，XXXXXX

XXXXXX

Dear Mr./Mrs.: ________（作者名）

I am a graduate student of Xx University（大學(xué)名）in China.I major in “__Educational Technology______”（您的專業(yè)）.Recently, I found one of your articles, titled “__________”（文章名）in---------(雜志名).I found it may help me achieve my goals in this research field.This would make a really positive contribution to my work.I would like to be able to read the full text of this article.The abstract makes the article sound very interesting.I know there is usually a fee required to obtain the full article from--------（雜志名）;however, as a student, my only income is a small scholarship which is about U S $30.00 per month.I wonder if you would consider sending me the full text by Email.Perhaps you would consider this as an act of friendshipbetween our two countries.Thank you for your kind consideration of this request.Sincerely: ___________（您的名字）

Dear...(author name): I am interested in your paper titled...published in...I would appreciate it if you could send me a soft copy of this paper.Thank you.Kind regards,...(your name)

Dear Dr XXX, I am a post-doctoral researcher at XX University.I have recently read your paper “__________”（文章名）.I am interested in trying your approach for detecting mutations in yeast.I was wondering if you would be kind enough to send me the pETTthMutS plasmid reported in your paper.My mailing address is: David Gresham XX University Beijing, 100101 China Kind regards，I'd appreciate a file(pdf)containing yourr article: Mutat Res.2004 Mar 22;547(1-2):41-7.Directly fishing out subtle mutations in genomic DNA with histidine-tagged ThermusthermophilusMutS.Wang J, Liu J.Thank you in advance Best wishes Liu

Dear Dr.XXX, I read with interest the abstract of your article in---------(雜志名), “__________”（文章名）Unfortunately, our library does not subscribe to---------(雜志名).I would be most appreciative if you could please send me a.doc or.pdf file of the article by e-mail.Thank you, ___________（你的名字）PHD candidate ___________（你的單位）

Dear Professor Aisha Siddiqa: I am a graduate student of Anhui Medical University in China.I major in pathology.And my project focuses on the treatment of breast cancer.I want to construct a vector pTRE-HER2 which your used in one of your paper(BMC Cancer ,vol8:129 DOI:10.1186/1471-2407-8-129).Constructing this vector seems to be a problem hard to get over for me.I have been working on it for a couple of months, but no progress.I am happy to find that your laboratory was the major source of the pTRE-HER2.Therefore, I am writing to explore the possibility of getting pTRE-HER2 from your laboratory.If you were kindly offering me the vector, it would be very helpful for me.Of course, the plasmid will never be used for commercial purpose.I will acknowledge the plasmid resource and cite your papers in all my publications based on the plasmid.Sure, I would like to sign any contract for the plasmid transfer.I am waiting for the vector to set up my experiment, so your early response will be greatly appreciated.Sincerely: Jingfeng Zhu My Email address is: 2217072522@qq.com

第二篇：向老外索要文章材料的英文書信范例

Dear Dr.viaud, I read with interest the abstract of your article in Mutation Research, “Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea”.Unfortunately, our library does not subscribe to Mutation Research.I would be most appreciative if you could

please send me a.doc or.pdf file of the article by e-mail.Thank you, Jiang PHD candidate Plant Disease Research Lab.,College of Plant Science and Technology, XX XX University,Wuhan, Hubei, 430070,P.R.China.Dear Dr.viaud, I'd appreciate a file(pdf)containing your article: Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea, which appeared in Molecular Microbiology.(2003 Dec;50(5): 1451-65.).Directly fishing out my thesis of the relative gene of the certain phytopathogenic fungus.Thank you in advance Best wishes Jiang PHD candidate Plant Disease Research Lab.,College of Plant Science and Technology, XX XX University,Wuhan, Hubei, 430070,P.R.China.Dear Dr.viaud, I am a post-doctoral researcher in Liu's lab at XX XX University.I have recently read your paper “Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea”, which appeared in Molecular Microbiology.(2003 Dec;50(5): 1451-65.).I am interested in trying your approach for detecting the same gene in another fungus.I was wondering if you would be kind enough to send me the pETTthMutS plasmid reported in your paper.My mailing address is:XX XX@XX XX.Plant Disease Research Lab.,College of Plant Science and Technology, XX XX University,Wuhan, Hubei, 430070,P.R.China.Kind regards, Jiang

Dear Dr.Andreas Schroeder, David Pauleen, Sid Huff

I read with interest the abstract of your article in Inderscience Publishers, “Emerging evidence on linkages between Knowledge Management(KM)governance and management strategy: the case of two organisations”.Unfortunately, our library does not subscribe to Inderscience Publishers.I would be most appreciative if you could.please send me a.doc or.pdf file of the article by e-mail: y_wenxi@126.com.Thank you in advance Best wishes Yaowei Business school of nankai university

Weijin Road, Nankai District, Tianjin 30071, P.R.China

在課題研究中，難免碰到比較好的參考文章，但是學(xué)校圖書館卻沒有購(gòu)買，此時(shí)向原作者索要原文是最好的辦法。下面是從其他博客轉(zhuǎn)引的索要文章參考模板，感謝原作者的辛勤勞動(dòng)。我在此做論文簡(jiǎn)要修改，以方便直接用,第一個(gè)索要原文，第二個(gè)索要文正涉及數(shù)據(jù)或軟件。Dear Dr.viaud，I read with interest the abstract of your article in Mutation Research, “Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea”.Unfortunately, our library does not subscribe to Mutation Research.I would be most appreciative if you could.please send me a.doc or.pdf file of the article by e-mail.Thank you, Dear Dr.viaud, I am a post-doctoral researcher in Liu's lab at XX XX University.I have recently read your paper “Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA arrays approaches in the phytopathogenic fungus Botrytis cinerea”, which appeared in Molecular Microbiology.(2003 Dec;50(5): 1451-65.).I am interested in trying your approach for detecting the same gene in another fungus.I was wondering if you would be kind enough to send me the pETTthMutS plasmid reported in your paper.參考：http://blog.sina.com.cn/s/blog_4a233a58010004zs.html Sincerely yours，Knowledge Management Research & Practice 7, 152-161(June 2009)| doi:10.1057/kmrp.2009.8 Export references

Concept maps: a technique for assessing knowledge manager learning needs Nigel Martin and John Rice

Overcoming knowledge dilemmas: governing the creation, sharing and use of knowledge resources

Authors: Frost, Jetta1;Morner, Michele2

Source: International Journal of Strategic Change Management, Volume 2, Numbers 2-3, 2 August

2010 , pp.172-199(28)

Publisher: Inderscience Publishers

Title: Improving competitive intelligence for knowledge management systems

Author: Kevin R.Parker, Philip S.Nitse

Address: Department of Computer Information Systems, College of Business, Idaho State

University, Campus Box 8020, Pocatello, ID 83209, USA.' Department of Marketing, College of

Business, Idaho State University Campus Box 8020, Pocatello, ID 83209, USA

Journal: International Journal of Internet and Enterprise Management 200545

Keywords: business intelligence;competitive intelligence;knowledge management;information

gathering;information needs;critical intelligence needs;key intelligence topics.DOI: 10.1504/IJIEM.2005.007229

Title: Dynamic hierarchies for business intelligence information retrieval

Author: Ryan C.LaBrie, Robert D.St.Louis

Address: School of Business and Economics, Seattle Pacific University, 3307 Third Avenue

West, Seattle, WA 98119-1950, USA, ' W.P.Carey School of Business, Arizona State University，Mail Stop 874606, Tempe, AZ 85287-4606, USA

Journal: International Journal of Internet and Enterprise Management 200523

DOI: 10.1504/IJIEM.2005.007228

Title: Web question answering: technology and applications to business intelligence

Author: Dmitri Roussinov, Jose Antonio Robles-Flores

Address: Department of Information Systems, W.P.Carey School of Business, Arizona State

University, P.O.Box 874606, Tempe, AZ 85287–4606, USA.' ESAN University, Lima, Peru and

Department of Information Systems, W.P.Carey School of Business, Arizona State University，P.O.Box 874606, Tempe, AZ 85287–4606, USA

Journal: International Journal of Internet and Enterprise Management 200562

Keywords: information retrieval;open domain question answering;web search;business

intelligence;natural language questions.DOI: 10.1504/IJIEM.2005.007230

Title: Parallel competitive intelligence processes in organisations

Author: Patrick T.Gibbons, John E.Prescott

Address: Division of Strategy and Information Systems, School of Accountancy and Business，Nanyang Technological University, Nanyang Avenue, 2263 Singapore.' Katz Graduate School of

Business, 252 Mervis Hall, University of Pittsburgh, Pittsburgh, PA 15260, USA

Journal: International Journal of Technology Management 1996178

Keywords: competitive intelligence;parallel processes;integrating mechanisms;competitive

information;information gathering;information analysis.DOI: 10.1504/IJTM.1996.025425

International Journal of Learning and Intellectual Capital

Issue:

Volume 1, Number 4 / 2004

Pages:

460482

URL:

Linking Options

Intellectual capital, competitive intelligence and the Economic Espionage Act

Chris Carr, G.Scott Erickson, Helen N.Rothberg

A1 Orfalea College of Business, California Polytechnic State University – San Luis Obispo, San

Luis Obispo, CA 93407, USA.A2 School of Business, Smiddy Hall, Ithaca College, Ithaca, NY 14850, USA.A3 School of Management, Marist College Poughkeepsie, NY 12601, USA

Knowledge convergence in an open innovation process

Authors: H, Jukka Bergman1;Karkkainen, Hannu2;Jantunen, Ari Jantunen3;Saksa, Juha-Matti Sak4

Source: International Journal of Knowledge Management Studies, Volume 4, Number 1, 2 December

2009 , pp.47-64(18)

Publisher: Inderscience Publishers

The reconfiguration of practical knowledge in competitive alliances Authors: Emberson, Caroline1;Storey, John2

Source: International Journal of Knowledge Management Studies, Volume 4, Number 2, 5 January

2010 , pp.114-131(18)

Publisher: Inderscience Publishers Dear Dr.viaud，I read with interest the abstract of your article in Mutation Research, “Cyclophilin A and calcineurin functions investigated by gene inactivation, cyclosporin A inhibition and cDNA

arrays approaches in the phytopathogenic fungus Botrytis cinerea”.Unfortunately, our library

does not subscribe to Mutation Research.I would be most appreciative if you could.please send

me a.doc or.pdf file of the article by e-mail.Thank you，The reconfiguration of practical knowledge in competitive alliances Authors: Emberson, Caroline1;Storey, John2

Source: International Journal of Knowledge Management Studies, Volume 4, Number 2, 5 January

2010 , pp.114-131(18)

Publisher: Inderscience Publishers

第三篇：向老外索要質(zhì)粒,菌種等的英文信

索要質(zhì)粒步驟：（提供建議）

1.先用PUBMED查找你所要的質(zhì)粒(不要選擇任何限制),一般來說,質(zhì)粒是不會(huì)寫入摘要或文題的.不過我個(gè)人覺得004km.cnmercial purpose.We will acknowledge the DNA resource and cite your papers in all our publications based on the DNA.We are also willing to put you into the author list that’s necessary.Sure, We would like to sign any contract for the DNA transfer.One of my graduates is waiting for the DNA to set up his experiment, so your early response will be greatly appreciated.Sincerely yours,

第四篇：如何向違約離職員工索要培訓(xùn)費(fèi)

如何向違約離職員工索要培訓(xùn)費(fèi)

企業(yè)在對(duì)員工出資培訓(xùn)后，通常會(huì)給員工設(shè)定一個(gè)服務(wù)期，有些員工在服務(wù)期未滿時(shí)便提前違約離職，但又不愿支付培訓(xùn)費(fèi)或違約金，對(duì)此很多企業(yè)都感到非常棘手。

1.法律規(guī)定違約離職員工應(yīng)當(dāng)支付違約金

我國(guó)《勞動(dòng)合同法》規(guī)定：“用人單位為勞動(dòng)者提供專項(xiàng)培訓(xùn)費(fèi)用，對(duì)其進(jìn)行專業(yè)技術(shù)培訓(xùn)的，可以與該勞動(dòng)者訂立協(xié)議，約定服務(wù)期。勞動(dòng)者違反服務(wù)期約定的，應(yīng)當(dāng)按照約定向用人單位支付違約金。違約金的數(shù)額不得超過用人單位提供的培訓(xùn)費(fèi)用。用人單位要求勞動(dòng)者支付的違約金不得超過服務(wù)期尚未履行部分所應(yīng)分?jǐn)偟呐嘤?xùn)費(fèi)用?！?/p>

一般來說，企業(yè)會(huì)給一些優(yōu)秀員工提供培訓(xùn)以提高他們的技能，使其更好地為企業(yè)做貢獻(xiàn)。在企業(yè)這一目標(biāo)實(shí)現(xiàn)的同時(shí)，員工也提高了個(gè)人的技能和水平及自身的市場(chǎng)價(jià)值。很多企業(yè)的骨干員工經(jīng)過培訓(xùn)后會(huì)更優(yōu)秀，可能很快就被競(jìng)爭(zhēng)對(duì)手以高薪挖走，對(duì)此企業(yè)通常會(huì)要求員工支付巨額的培訓(xùn)費(fèi)用，但員工往往不愿意支付，這就產(chǎn)生了糾紛。

案例

——企業(yè)能拿回巨額培訓(xùn)費(fèi)嗎？——

某企業(yè)的一位員工在國(guó)外接受了一年的公費(fèi)培訓(xùn)，回國(guó)后工作不到3個(gè)月就被一家競(jìng)爭(zhēng)對(duì)手挖走了。對(duì)此，企業(yè)認(rèn)為該員工應(yīng)支付企業(yè)用在他身上的培訓(xùn)費(fèi)用共計(jì)20萬(wàn)元，否則就不能離職，而員工表示自己沒錢支付，便強(qiáng)行離開了。

企業(yè)不甘心損失20萬(wàn)元的培訓(xùn)費(fèi)，于是向勞動(dòng)爭(zhēng)議仲裁委員會(huì)提起仲裁。開庭后，仲裁員先進(jìn)行調(diào)解，對(duì)企業(yè)說：“這個(gè)情況我們已經(jīng)調(diào)查清楚了，這位員工確實(shí)違約了，但他又確實(shí)賠不起20萬(wàn)元。不如我們給他做做工作，看看他能拿出多少錢來，就拿出多少來支付

給你們，你們看行不行？”對(duì)此，企業(yè)表示不同意。這時(shí)，仲裁員又說：“如果你覺得虧，不進(jìn)行調(diào)解，那就直接做裁決，讓他賠償20萬(wàn)元。但企業(yè)基本上也拿不到，因?yàn)樗麍?jiān)持說沒錢，就只能去法院申請(qǐng)強(qiáng)制執(zhí)行。但這個(gè)員工一是沒結(jié)婚，跟父母住在一起，很難界定他有什么個(gè)人財(cái)產(chǎn)；二是他沒有個(gè)人存款，即使有，企業(yè)沒有他的賬號(hào)，法院也無(wú)法執(zhí)行?！?/p>

聽完仲裁員的解釋，企業(yè)負(fù)責(zé)人也犯了愁，到底怎樣才能拿回巨額的培訓(xùn)費(fèi)呢？

在這一案例中，仲裁員的這種解決方式會(huì)對(duì)企業(yè)造成很大的負(fù)面影響：一方面，企業(yè)無(wú)法獲得足夠的賠償，經(jīng)濟(jì)上蒙受了損失；另一方面，企業(yè)的其他員工看到只要支付很少的賠償金就可以離職，很可能會(huì)效仿，況且企業(yè)以后還會(huì)大量培訓(xùn)員工。

2.企業(yè)向違約離職員工索要違約金的有效方式

事實(shí)上，在向違約離職員工索要違約金時(shí)，企業(yè)可以采取一系列行之有效的方法進(jìn)行處理：

第一步，企業(yè)可以起訴違約離職員工，要求其支付違約金，同時(shí)不接受減少違約金的調(diào)解，而是要求仲裁庭做出員工必須如數(shù)賠償培訓(xùn)費(fèi)的裁決。

第二步，拿到裁決后，企業(yè)可以把裁決書的內(nèi)容在內(nèi)部做通告，并附上一個(gè)通知，說明本公司的員工××違約以后不支付違約金，公司提起了仲裁并且勝訴了，結(jié)果是員工必須如數(shù)支付違約金。這樣，能夠讓企業(yè)的其他員工感受到企業(yè)鮮明的態(tài)度和決心。

第三步，如果員工拒不支付且無(wú)法強(qiáng)制執(zhí)行，企業(yè)可以在申請(qǐng)強(qiáng)制執(zhí)行的同時(shí)，請(qǐng)法院做出“中止執(zhí)行”的裁定，即員工目前沒有財(cái)產(chǎn)可供執(zhí)行，一旦企業(yè)發(fā)現(xiàn)他擁有財(cái)產(chǎn)，就可以到法院申請(qǐng)恢復(fù)執(zhí)行。換言之，這筆違約金會(huì)成為該員工終身背負(fù)的一筆債務(wù)。

第四步，企業(yè)可以與員工約談，說明企業(yè)已經(jīng)掌握了他在新單位

工作的情況和薪酬待遇，目前可以不要求對(duì)方一次性支付，而是簽署一份分期還款協(xié)議，以這種方式支付培訓(xùn)費(fèi)。

第五步，如果員工拒絕簽署分期還款協(xié)議，企業(yè)可以將員工新就職的單位提供給法院，從法律上講，法院可以向新單位發(fā)出協(xié)助執(zhí)行通知書，責(zé)成新單位協(xié)助執(zhí)行，內(nèi)容大約是“該員工目前欠原單位違約金多少元，現(xiàn)要求你單位協(xié)助執(zhí)行，每月從該員工的工資中扣除多少元交給法院，由法院轉(zhuǎn)交給原單位”等。

經(jīng)過上述步驟，員工在分析利弊得失后，通常會(huì)同意簽署分期還款協(xié)議。最后企業(yè)不但能拿回全部培訓(xùn)費(fèi)，更讓其他員工看到企業(yè)有足夠的法律手段追回培訓(xùn)費(fèi)，震懾其他員工，打消其他員工跳槽的念頭。由此可見，企業(yè)對(duì)于員工違約離職行為必須進(jìn)行堅(jiān)決而有效的處理，拿回培訓(xùn)費(fèi)只是其中一個(gè)直接目的，更深層的目的是遏制其他員工的違約行為，讓員工安心履行完服務(wù)期。

第五篇：寫信向國(guó)外科研人員索要試驗(yàn)所需材料

范文：Dear...., I am working on......(工作背景).And one of my projects focuses on........(工作內(nèi)容)Isolating ROG cDNA seems to be a problem hard to get over for us（為什么要要）.One of my graduates has been working on it for a couple of months, but no progress（一定以導(dǎo)師和教授口氣寫，學(xué)生寫人家可能不愿意給）.We are happy to find that your laboratory was the major source of the ROG cDNA in many publications.Therefore, I am writing to explore the possibility of getting ROG cDNA from your laboratory.If you were kindly offering us the DNA(pCI-ROG), it would be very helpful for us.Of course, the DNA will never be used for commercial purpose.We will acknowledge the DNA resource and cite your papers in all our publications based on the DNA.We are also willing to put you into the author list that’s necessary.Sure, We would like to sign any contract for the DNA transfer.[BR]One of my graduates is waiting for the DNA to set up his experiment, so your early response will be greatly appreciated.Sincerely yours, [BR]

注：

收到質(zhì)粒后,一定要回去一封感謝信.并再次重申不會(huì)用于商業(yè)或臨床目的.Dear Professor Bruss V:

I am working on immunology.And one of my projects focuses on the treatment of HBV.We want to construct a vector pHBV1.5 which your used in one of your paper(Proc.Natl.Acad.Sci.USA,vol88:1059-1063).Construct this vector seems to be a problem hard to get over for us.One of my graduates has been working on it for a couple of months, but no progress.We are happy to find that your laboratory was the major source of the pHBV1.5.Therefore, I am writing to explore the possibility of getting pHBV1.5 from your laboratory.If you were kindly offering us the vector, it would be very helpful for us.Of course, the plasmid will never be used for commercial purpose.We will acknowledge the plasmid resource and cite your papers in all our publications based on the plasmid.Sure, We would like to sign any contract for the plasmid transfer.One of my graduates is waiting for the vector to set up his experiment, so your early response will be greatly appreciated.結(jié)果給我寄過來了,他是用濾紙寄的,先用500ng質(zhì)粒溶解在1ulTE里,然后滴在濾紙上用塑料袋封閉,用的時(shí)候同樣使用50ulTE將質(zhì)粒溶解在EP管中,短暫離心后上清即可用于細(xì)菌轉(zhuǎn)化.他是用空寄的,所以不需要通過海關(guān),一般半個(gè)月就到了.